Record Information
Version1.0
StatusDetected and Quantified
Creation Date2021-11-17 23:47:46 UTC
Update Date2022-08-31 17:37:50 UTC
Metabolite IDMMDBc0029943
Metabolite Identification
Common Name(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate
Description2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylic acid (SHCHC) is the first identified intermediate in the menaquinone biosynthetic pathway. This pathway requires two reactions. They are the decarboxylation of alpha-ketoglutarate by an alpha-ketoglutarate decarboxylase, which results in the formation of succinic semialdehyde-thiamine PPi (TPP) anion, and the addition of the succinic semialdehyde-TPP anion to isochorismate carried out by the enzyme SHCHC synthase. In E. coli, addition of succinic semialdehyde-TPP anion (from 2-oxoglutarate) to isochorismate results in the formation of 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC). In the subsequent reaction, the pyruvoyl group is eliminated, resulting in the prearomatic compound (1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate (SHCHC). This is then aromatized to the benzenoid aromatic compound o-succinylbenzoate (OSB) and used as the framework for the construction of the rest of the menaquinone molecule. In this pathway, prenylation in the last step leads to the formation of 1,4-dihydroxy-2-naphtoic acid (DHNA). Subsequently OSB and DHNA formed in this pathway are incorporated into the naphthoquinone ring of menaquinone. SEPHCHC is an unstable compound and in mildly basic solutions, spontaneously undergoes a 2,5-elimination reaction resulting in the formation SHCHC and pyruvate. But the in vivo conversion of SEPHCHC to SHCHC is carried out by SHCHC synthase MenH. Dehydration from SHCHC by the enzyme OSB synthase (MenC) leads to the formation of the benzenoid aromatic compound OSB. The conversion of the benzenoid aromatic compound OSB to the naphthalenoid aromatic compound DHNA is catalyzed by the enzyme OSB-CoA synthetase (MenE). The process has been shown to have an absolute requirement for ATP and CoA. OSB-CoA is suggested as an intermediate. During the formation of OSB-CoA, ATP is hydrolyzed to AMP and PPi.
Structure
Synonyms
ValueSource
(1R,6R)-6-Hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylateChEBI
(1R,6R)-6-Hydroxy-2-succinyl-cyclohexa-2,4-diene-1-carboxylic acidGenerator
(1R,6R)-6-Hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylic acidGenerator
Molecular FormulaC11H10O6
Average Mass238.196
Monoisotopic Mass238.048835202
IUPAC Name(1R,6R)-2-(3-carboxylatopropanoyl)-6-hydroxycyclohexa-2,4-diene-1-carboxylate
Traditional Name(1R,6R)-2-(3-carboxylatopropanoyl)-6-hydroxycyclohexa-2,4-diene-1-carboxylate
CAS Registry NumberNot Available
SMILES
[H][C@@]1(O)C=CC=C(C(=O)CCC([O-])=O)[C@@]1([H])C([O-])=O
InChI Identifier
InChI=1S/C11H12O6/c12-7(4-5-9(14)15)6-2-1-3-8(13)10(6)11(16)17/h1-3,8,10,13H,4-5H2,(H,14,15)(H,16,17)/p-2/t8-,10-/m1/s1
InChI KeyQJYRAJSESKVEAE-PSASIEDQSA-L